3 h]acetyl-coa (Hartmann Analytics)
90
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Hartmann Analytics
3 h]acetyl-coa
3 H]Acetyl Coa, supplied by Hartmann Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3+h%5Dacetyl-coa/%CE%B3++32+p+atp/pmc07401105-692-52-66
Average 90 stars, based on 1 article reviews
3 H]Acetyl Coa, supplied by Hartmann Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3+h%5Dacetyl-coa/%CE%B3++32+p+atp/pmc07401105-692-52-66
Average 90 stars, based on 1 article reviews
3 h]acetyl-coa - by Bioz Stars,
2026-10
90/100 stars
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Activity Assay:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Purification:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Labeling:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 In Vitro:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Incubation:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Binding Assay:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Modification:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Staining:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 Negative Control:Article Title: Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling Article Snippet: Additionally, the auto-Kat activity of AtNAA70 was quantified by determination of incorporated [ 3 H] label with a Tri-Carb 2810TR scintillation counter (PerkinElmer) after incubation of 20 μg purified His-MBP-AtNAA70 at 37°C in acetylation buffer (50 mM Tris-HCl (pH 8.5), 10% glycerol, 1 mM EDTA) containing 60 μM [ 3 Article Title: NAA50 Is an Enzymatically Active N α -Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses Article Snippet: To measure the NAT activity of AtNAA50, 4.5 μg (150 pmol) of purified enzyme was mixed with 0.2 m m of a custom-made peptide (GeneCust), 0.2% (w/v) BSA in acetylation buffer (50 m m Tris-HCl, pH 7.5, 8 m m EDTA, and 1 m m DTT), and 45 μ m [ 3 |
![AtNAA70 possesses N ε -acetylation activity on internal Lys residues. (A) MS spectrum of the AtNAA70 internal LIWKAEALAKNWGCR peptide with three distinct isotope distributions corresponding to the peptide with A 1 ) two heavy acetylations (d3-Acetyl, chemical modification), A 2 ) a mix of heavy and light acetylations (d3-Acetyl/Ac), A 3 ) two light acetylations (endogenous). The MS signal extractions displayed in A 1 to A 3 are used to determine the relative abundance of each form (unmodified peptide: 84%, partial Lys acetylation (on Lys 217 or 223): 14%, Acetylation of both Lys 217 and 233: 2%. (B) Annotated synthetic MSMS of the peptide (combination of spectra with precursor mass of 953.45 ± 0.5 Da). (C) Immunological detection of ac-Lys on AtNAA70 in absence (–) or presence (+) of acetyl-CoA for indicated time points using an N ε -acLys specific antiserum. Staining of AtNAA70 with amido black serves as loading control. (D) Determination of AtNAA70 auto-Kat activity by incorporation of [ 3 H]-acetyl moieties into purified AtNAA70 (black circles) over time. Incubation of [ 3 H]-acetyl-CoA with heat inactivated AtNAA70 (white circles) for 60 min served as a negative control. ( N = 3)](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2087/pmc04692087/pmc04692087__pmic0015-2426-f5.jpg)